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Notwithstanding The range of techniques that have been introduced to beat artefacts relevant to information compositionality, they've not been adopted for your human microbiome investigation. Of Take note, none of the clinical trials revealed in the course of the past year (besides those exclusively addressing the compositionality problem [five, six]) utilized quantitative microbiome profiling. In this article, we present how quantitative PCR (qPCR)-centered bacterial enumeration is often built-in to NGS pipelines to supply a possible approach to estimate absolute abundances from NGS knowledge, and therefore advertise using quantitative microbiome profiling in the sphere of human microbiome.
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qPCR is Charge-productive and accessible as being the laboratory options, machinery and reagents are just like those desired for getting ready the NGS libraries.
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